Jagielka ruled out of Montenegro match
Jagielka, who has been replaced by Bolton Wanderers centre-back Gary Cahill, had been in contention to start the match at Wembley after impressive displays in England's last two matches against Bulgaria and Switzerland.
But the Everton star missed a training game at Arsenal's London Colney complex after experiencing tightness in his hamstring during the previous session.
A scan confirmed Jagielka would not be fit to face Montenegro, with England coach Fabio Capello now likely to restore Rio Ferdinand and John Terry to the centre of his defence after the pair missed the team's last two qualifiers.
Meanwhile, Manchester City midfielder Gareth Barry joined up with the England squad today after being given additional time at home due to personal reasons.