Barcelona superstar Messi, Manchester City striker Aguero as well as Gonzalo Higuain, the Napoli frontman, were all called to the squad for the games against Peru on 11 October in Buenos Aires and Uruguay in Montevideo four days later.
Barcelona midfielder Javier Mascherano was named in the squad although he remains a doubt with a thigh injury.
However, Juventus striker Carlos Tevez and Paris Saint-Germain midfielder Javier Pastore were overlooked by Sabella.
Argentina made sure of their place in the World Cup by beating Paraguay 5-2 last month.
They have 29 points in the South American qualifying zone ahead of Colombia (26 pts), Chile (24), Ecuador and Uruguay (22).
The top four qualify for the Brazil showpiece with the fifth-placed nation facing a play-off.
Goalkeepers: Sergio Romero (Monaco/FRA), Mariano Andujar (Catania/ITA)
Defenders: Hugo Campagnaro (Inter Milan/ITA), Federico Fernandez (Napoli/ITA), Pablo Zabaleta (Manchester City/ENG), Fabricio Coloccini (Newcastle/ENG), Marcos Rojo (Sporting Lisbon/POR), Ezequiel Garay (Benfica/POR), Cristian Ansaldi (Zenit St-Petersburg/RUS), Jose Basanta (Monterrey/MEX)
Midfielders: Javier Mascherano (Barcelona/ESP), Angel Di Maria (Real Madrid/ESP), Ever Banega (Valencia/ESP), Augusto Fernandez (Celta Vigo/ESP), Ricardo Alvarez (Inter Milan/ITA), Lucas Biglia (Lazio/ITA), Jose Sosa (Metallist/UKR), Erik Lamela (Tottenham/ENG).