With John Terry now retired from international football, there has been plenty of speculation about who the fourth central defender would be.
Tottenham Hotspur's Steven Caulker was considered to be a contender but he was named in Stuart Pearce's U-21 squad on Wednesday.
Stoke City's Shawcross has therefore been picked for only the second time, having failed to win a cap when he was chosen for the friendly against Egypt by Fabio Capello in March 2010.
Arsenal's Gibbs, who has two caps from friendly encounters against Hungary and France, has replaced Ryan Bertrand in the squad and has been rewarded for his excellent performances for Arsenal this season.
Like Caulker, Jack Butland has been released to the U-21s for their European Championship play-off with Serbia, leading to Celtic's Forster becoming a rare England selection from the Scottish Premier League.
Goalkeepers: Fraser Forster (Celtic), Joe Hart (Manchester City), John Ruddy (Norwich City)
Defenders: Leighton Baines (Everton), Gary Cahill (Chelsea), Ashley Cole (Chelsea), Kieran Gibbs (Arsenal), Phil Jagielka (Everton), Glen Johnson (Liverpool), Joleon Lescott (Manchester City), Ryan Shawcross (Stoke City), Kyle Walker (Tottenham Hotspur)
Midfielders: Michael Carrick (Manchester United), Tom Cleverley (Manchester United), Steven Gerrard (Liverpool), Adam Johnson (Sunderland), Aaron Lennon (Tottenham Hotspur), Frank Lampard (Chelsea), James Milner (Manchester City), Alex Oxlade-Chamberlain (Arsenal), Theo Walcott (Arsenal)
Forwards: Andy Carroll (West Ham United), Jermain Defoe (Tottenham Hotspur), Wayne Rooney (Manchester United), Danny Welbeck (Manchester United)