Ghana's Black Stars will play Germany in Fortaleza on 21 June in Group G, having also drawn Portugal and USA, meaning Schalke's attacking midfielder Kevin Prince could be up against Bayern Munich centre-back Jerome.
This is not the first time the brothers have met at a World Cup as they both played when Germany beat Ghana 1-0 at the 2010 World Cup in South Africa in the group stages - the first time brothers had played on opposite teams at a World Cup.
"Brother, it's time again...that's how beautiful life is! Can't wait...love u bro!!!" Kevin-Prince wrote on his Twitter account.
"I'm happy with that," Boateng told Sport Bild. "You can see that dreams can come true. I am looking to another game against my brother at the World Cup. And also against my team-mate Jermaine Jones, he's simply great."