Switzerland and Slovakia seized Europe's last two automatic berths in South Africa, while Portugal, Greece, Slovenia and Ukraine made the play-offs.
Peter Crouch scored a brace to boost his chances of a place at South Africa 2010 as England ended their qualifying campaign by beating Belarus 3-0.
Slovenia cruised to victory away to San Marino, but were forced to settle for a place in the play-offs courtesy of Slovakia's 1-0 success in Poland.
Slovakia captain Robert Vittek has vowed that "qualification will be ours" after suffering a setback in their South Africa 2010 bid against Slovenia.
Switzerland, Greece, Slovakia and Slovenia head into Wednesday's final qualifiers hoping to claim the two remaining direct tickets to South Africa.
Cologne defender Miso Brecko tells FIFA.com all about Slovenia's plans to spoil the Slovak party ahead of their qualifying clash in Bratislava.