Bulgaria ended their campaign with a 6-2 victory over Group 8 tailenders Georgia in Vaduz, with star man Dimitar Berbatov scoring a hat-trick.
Switzerland and Slovakia seized Europe's last two automatic berths in South Africa, while Portugal, Greece, Slovenia and Ukraine made the play-offs.
Switzerland, Greece, Slovakia and Slovenia head into Wednesday's final qualifiers hoping to claim the two remaining direct tickets to South Africa.
Italy have qualified for South Africa 2010 and will start the competition amongst the favourites, but this was far from a comfortable cruise to the finals.
Italy, Germany, Denmark and Serbia all sealed their places at South Africa 2010 with positive results during Saturday's nervy round of qualifiers.
Andrija Delibasic scored the winner on his debut as Montenegro earned their maiden South Africa 2010 qualifying triumph with a defeat of Georgia.