With former FIFA World Cup-winners Uruguay, Italy and England all competing in Group D, the outcome looks tough to call, while Costa Rica will be out to perform a heroic shock in trying to negotiate past these football giants.
In the wake of Uruguay becoming the last of the 32 teams to qualify for Brazil 2014, FIFA.com looks at the statistics behind their successful campaign.
FIFA.com spoke with Diego Forlan, the South Africa 2010 Golden Ball winner and the third generation of his family to play for Uruguay, who was brought up immersed in Celeste tradition.
Despite a tough 5-0 loss to Uruguay, Jordan were able to look at the positives from the Asian nation's first-ever intercontinental play-off berth for a chance to qualify for a World Cup™.
FIFA.com previews the FIFA World Cup™ play-off between Jordan and Uruguay with the west Asians looking to make the most of home advantage on Wednesday against the highly-regarded South Africa 2010 semi-finalists.
A single point is all that is required for Colombia to guarantee their place at the 2014 FIFA World Cup Brazil™, while a point may also be enough for Argentina if either Ecuador or Uruguay fail to win their games.