Having enjoyed enthralling runs to the semi-finals, both Uruguay and Germany will be hoping to end the tournament on a high in the match for third place at Port Elizabeth Stadium on Saturday.
In a repeat of the UEFA EURO 2008 final, Spain and Germany will lock horns on Wednesday night for the right to join the Netherlands in the Final of South Africa 2010.
La Celeste are the sole surviving South American representatives and will aim to prevent an all-European final by beating the Netherlands, while Spain and Germany reprise their EURO 2008 final.
Argentina will aim to avenge their 2006 defeat by Germany when the teams meet again in the quarter-finals, while Paraguay's defence faces a daunting test against Spain.
Brazil and the Netherlands meet once again on the world stage and Uruguay face Africa's remaining hope Ghana in the opening two quarter-final matches on Friday.
Spain, many observers' tip for the title, face a tough last 16 test against neighbours Portugal, while Group F winners Paraguay take on Asia's sole remaining representatives, Japan.
All the pressure will be on the Netherlands and Brazil when they take on Slovakia and Chile, teams who have already achieved a measure of success in reaching the last 16.
Two epic match-ups mark the second day of Round of 16 action with England meeting old foes Germany, and Argentina reprising their 2006 encounter against Mexico.
The 2010 FIFA World Cup will enter the first round of knockout matches with Uruguay and Korea Republic opening proceedings followed by USA meeting Ghana.
European champions Spain have work to do to book their place in the Round of 16 when the curtain falls on Groups G and H with three places still up for grabs.
Italy are living dangerously as they enter their final Group F match against Slovakia still seeking a first win, while in Group E Denmark look to leapfrog Japan.
Everything is still to play for in Groups C and D where all eight teams are still in with a shout – and England and Germany risk an early exit.
Uruguay and Argentina begin the day as leaders of Group A and Group B respectively, but with no-one yet assured of a last 16 place or consigned to elimination, there is still plenty to play for.
Although Spain were widely backed to take Group H by storm, it is Chile and Switzerland who will battle for top spot in the section after the duo both recorded wins in their initial outings.
Brazil will aim to turn on the style in a highly anticipated clash with Côte d'Ivoire while Italy take on minnows New Zealand in one of two Group F contests.
Group E rivals Cameroon and Denmark have no margin for error in their meeting in Durban and the same goes for an Australia side facing Ghana.
Three more pivotal matches mark Day 8 with Serbia and England having little room for error, while Germany look to maintain their remarkable start.
Argentina and Korea Republic will cross paths for the second time in FIFA World Cup history on Thursday knowing that whoever wins in their Group B meeting will take a giant stride towards the Round of 16.
Generally regarded as the favourites for the 2010 FIFA World Cup South Africa™, Spain’s first appearance in this year’s tournament is Wednesday’s major highlight.
The only country to have won the FIFA World Cup™ outside their own continent, five-time champions Brazil take their opening bow at South Africa 2010 against Group G outsiders Korea DPR.
Day 4 at South Africa 2010 sees an all-European affair between the Netherlands and Denmark, while Italy take on Paraguay and Asian hopefuls Japan meet Cameroon.
The highlight of Sunday’s three games is the Group D meeting between three-time world champions Germany and an Australia side looking to build on the progress they made at Germany 2006.
Diego Maradona makes his FIFA World Cup debut as a coach with Argentina against Group B rivals Nigeria while Group C offers an England-USA rematch.
A new chapter will be etched into football history today as the first FIFA World Cup finals to be held on African soil get under way at Soccer City in Johannesburg.
FIFA.com wants to know what was your favourite moment of South Africa 2010.
Just minutes after learning of his award, the adidas Golden Ball winner for the 2010 FIFA World Cup granted an exclusive interview to FIFA.com.