Blanco had made his intentions known before the match, in which he went on in the closing moments for Fernando Arce to make his 100th appearance for the national team.
The 35-year-old, who scored 34 goals for Mexico in his international career, admitted he would have liked to make his final appearance at famed Azteca stadium, but said it was time to make way for younger players.
"The decision was already made," he said. "I'm content. I believe that my time in the selection is done," said the talented player who is beloved by Mexican fans.
Blanco made his debut for Mexico on 1 February 1995, in a friendly against Uruguay. He played in two FIFA World Cups, in France in 1998 and Korea Republic and Japan in 2002, and helped Mexico win the FIFA Confederations Cup in 1999. He is currently playing for the Chicago Fire in the USA's Major League Soccer.