The two kick off the global spectacle at Soccer City having met three times previously, with the visitors holding a 2-1 advantage.
Javier Aguirre's team have made it through the first phase of the last four FIFA World Cups, making them a real threat for Bafana Bafana in a Group A that also features France and Uruguay.
"It will be a big tough game, you know how they play," Mosimane said. "They play 3-4-3 and switch to 3-5-2, and they are very difficult to contain under those circumstances.
"Their system is confusing to their opponents and they are not afraid to throw men forward."