The Real Madrid defender has only just returned to training after five months out of action, however, he was a stand-out in a scrappy Portuguese qualifying campaign to ensure his place.
Coach Carlos Queiroz had previously indicated he would name his 23-man squad for the finals tomorrow, but chose to add another name to his selection tonight after admitting he had "one or two" doubts about the fitness of certain players.
"Things can happen at a training camp and we want to be sure the ones we take are in the best shape," the former Real Madrid boss said.
"The World Cup will take place when players are usually on holiday, it will be important to prepare them correctly so they can contribute as best as possible."
The biggest surprise of the squad was the inclusion of back-up goalkeepers Beto and Daniel Fernandes who did not play during qualifying.
Portugal reached the semi-finals of the FIFA World Cup in Germany four years ago, but face a difficult task to get out of their group in South Africa after being draw against Brazil, Ivory Coast and Korea DPR.
Portugal's provisional squad:
Goalkeepers: Beto (Porto), Daniel Fernandes (Iraklis), Eduardo (Braga).
Defenders: Fabio Coentrao (Benfica), Paulo Ferreira (Chelsea), Ricardo Carvalho (Chelsea), Ze Castro (Deportivo La Coruna), Bruno Alves (Porto), Rolando (Porto), Ricardo Costa (Lille), Duda (Malaga), Pepe (Real Madrid), Miguel (Valencia).
Midfielders: Tiago (Atletico Madrid), Deco (Chelsea), Raul Meireles (Porto), Nani (Manchester United), Miguel Veloso (Sporting Lisbon), Pedro Mendes (Sporting Lisbon).
Forwards: Simao Sabrosa (Atletico Madrid), Hugo Almeida (Werder Bremen), Liedson (Sporting Lisbon), Cristiano Ronaldo (Real Madrid), Danny (Zenit St Petersburg).
* Please note that squad lists only become official after they have been submitted to and announced by FIFA.