Romania coach Razvan Lucescu had named Mutu in his squad for the France game and the qualifier against Austria four days later despite the forward missing his club Fiorentina's Serie A game at the weekend.
Despite taking part in training on Wednesday morning he was later declared unfit. "The medical test conducted on Wednesday shows that Adrian Mutu's right thigh muscle problem has not improved, which rules him out of the games against France and Austria," the Romania Football Federation announced.
He is due to be replaced by Gheorghe Bucur, a striker with Romania first division side FC Timisoara. Romania's bid to reach the 2010 finals in South Africa was fatally undermined by three defeats in their opening six matches.