After Chile lost 4-0 to Japan and drew 1-1 with Belgium in that tournament, Bielsa has called on players including Mark Gonzalez - who went down to the Spanish second division with Real Betis at the weekend - and Mexican-based duo Humberto Suazo and Hector Mancilla, Villarreal forward Matias Fernandez and Bayer Leverkusen defender Arturo Vidal.
The Argentinian boss left out seven players who took part in the Kirin Cup - Universidad de Chile duo Manuel Iturra and Jose Rojas, midfielders Jose Pedro Fuenzalida (O'Higgins) and Braulio Leal (Union Espanola), and forwards Daud Gazale (Colo Colo), Esteban Paredes (Santiago Morning) and Edson Puch (Iquique).
Chile lie third in the South American qualifying zone with 20 points, one point behind second-placed Brazil and four adrift of leaders Paraguay. Chile travel to Paraguay on Saturday before they take on Bolivia in Santiago on 10 June.
Defenders: Pablo Contreras (PAOK), Waldo Ponce (Velez Sarsfield), Arturo Vidal (Bayer Leverkusen), Gonzalo Jara (Colo Colo), Ismael Fuentes (Atlas)
Midfielders: Marco Estrada (Universidad de Chile), Roberto Cereceda, Rodrigo Millar (both Colo Colo), Gary Medel (Universidad Catolica), Jorge Valdivia (Al Ain), Carlos Carmona (Reggina), Mauricio Isla (Udinese), Rodrigo Tello (Besiktas), Matias Fernandez (Villarreal)
Forwards: Fabian Orellana (Audax Italiano), Jean Beausejour (America), Mark Gonzalez (Betis), Alexis Sanchez (Udinese), Hector Mancilla (Toluca), Humberto Suazo (Monterrey)