Lampard said in a statement released by his management company: "I have taken the decision to retire from international football. It has been a very tough decision for me to make which is why I have given it so much thought since the World Cup."
Lampard ended his 13-year stay at Chelsea in the summer and is currently on loan at Manchester City from Major League Soccer side New York City.
He added: "I have always been exceptionally proud and honoured to represent my country and have to say looking back I have enjoyed every minute of wearing the England shirt.
"Due to my club career now going in a different direction it is of utmost importance for me to consider my family first - also, to concentrate on how I can perform consistently to the best of my abilities over my next few years in club football.
"It is now the time to move forward and I feel very confident that, with Roy Hodgson in charge, the young players that we have coming through and the changes that are being made throughout the development of the youth system in this country, we will have success in the future and a team that this country deserves.
"I would like to thank all the managers that I have worked under, all the players that I have been fortunate enough to play alongside, and all the England fans for their support, not only at home, but particularly on their travels with the team around the world.
"I now look forward to continuing to support the team as a fan and wish them every success for the upcoming campaign towards France 2016."
Lampard made his England debut in 1999 at the age of 21, but it was not until UEFA EURO 2004 that he played in a major championships. Since then he has appeared at every major tournament England have qualified for, including three FIFA World Cup™ finals. He is the joint sixth most-capped player in England history, level with Bobby Charlton, while his 29 international goals puts him joint ninth on the country's list of top goalscorers.
The Londoner's decision comes little more than a month after long-time central midfield partner and England captain Steven Gerrard ended his England career, marking the end of an era for the national team. Lampard's final England game came against Costa Rica at the World Cup in Brazil, when he captained the already-eliminated team in a 0-0 draw.