Manchester United fell 3-2 to Yokohama F Marinos to suffer their second loss in three games under new manager David Moyes.
The English champions fell behind in the very first minute, but they recovered to lead 2-1 heading into the second half thanks to Jesse Lindgard's strike and an own-goal. However, Fabio Aguiar headed the equaliser before Yoshihito Fujita's winner three minutes from time removed further gloss from United's pre-season Asian tour.
So far under Moyes, United have lost 1-0 in Bangkok and won 5-1 in Sydney, and they got off to a horror start in hot and humid conditions in Yokohama. The game was just one minute old when Brazilian striker Marquinhos latched on to a poor clearance from United goalkeeper David de Gea to fire the hosts in front from inside the box.
The setback stung the Premier League champions and talented Belgian teen Adnan Januzaj nearly put them level with a crisp strike which was just off-target.
Januzaj, 18, then got back to head a chance off the line, but it was new signing Wilfried Zaha who orchestrated United's opener when he drifted past his man into the box. The ex-Crystal Palace man's low cross caused mayhem and it was Lindgard on hand to net his third goal in two games, after Saturday's brace against the A-League All-Stars.
Yokohama handed United the lead when Januzaj, United's reserve team player of last season, fired a free-kick which came off the unfortunate Masakazu Tashiro and over the line.
But the hosts were back on terms after the break when de Gea failed to deal with a corner and Brazilian defender Fabio Aguiar's header moved the score to 2-2.
Shinji Kagawa drew a huge cheer when he came on just after the hour, and United's Japanese playmaker nearly raised the roof when he got clear, but his shot failed to beat the De Gea.
Ashley Young hit one into the side-netting, but it was Yokohama who had the final word when Fujita swept home the winning goal in the 87th minute.
Moyes, facing the stiff challenge of following Sir Alex Ferguson's 27-year, trophy-laden reign, now leads his team west for a game against Japan's Cerezo Osaka on Friday.