Jubilo Iwata defender Yuichi Komano, a member of Japan's 2010 FIFA World Cup™ team, returns to the Blue Samurai after recovering from a shoulder injury sustained last October, while Cerezo Osaka and Japan U-22 midfielder Hiroshi Kiyotake won a first call-up to the senior side.
"This will be an important game for us before going into the World Cup qualifiers," said Zaccheroni.
Since the Italian's arrival almost a year ago, Japan have played Korea Republic twice - drawing 0-0 in Seoul last October and winning on penalties after a 2-2 extra-time draw in the 2011 AFC Asian Cup semi-finals in January.
"Korea [Republic] are a wonderful side and whenever we play against them, it is always heated," Zaccheroni added.
"A team can grow through a tough serious game, and this will be a good one. It has been a while since we last played a game like that. I am hoping we can get our feeling back with this game before going into the qualifiers."
Japan are also scheduled to play Korea DPR at home on 2 September as final preparation for the 2014 FIFA World Cup qualifiers.
Goalkeepers: Eiji Kawashima (Lierse SK), Masaaki Higashiguchi (Albirex Niigata), Shusaku Nishikawa (Sanfrecce Hiroshima)
Defenders: Yuichi Komano (Jubilo Iwata), Yasuyuki Konno (FC Tokyo), Yuzo Kurihara (Yokohama F Marinos), Masahiko Inoha (Hajduk Split), Tomoaki Makino (Cologne), Atsuto Uchida (Schalke), Maya Yoshida (VVV Venlo)
Midfielders: Yasuhito Endo (Gamba Osaka), Yuki Abe (Leicester City), Makoto Hasebe (Wolfsburg), Hajime Hosogai (Augsburg), Akihiro Ienaga (Real Mallorca), Yosuke Kashiwagi (Urawa Reds)
Forwards: Daisuke Matsui (Dijon), Tadanari Lee (Sanfrecce Hiroshima), Shinji Okazaki (Stuttgart), Keisuke Honda (CSKA Moscow), Takayuki Morimoto (Novara), Shinji Kagawa (Borussia Dortmund), Hiroshi Kiyotake (Cerezo Osaka)