Juninho struck a 90th-minute winner as strugglers Atletico Goianiense defeated Campeonato leaders Fluminense 2-1.
Washington put the pace-setters in front after 22 minutes before William levelled seven minutes later and Juninho sealed the shock result in the dying seconds.
Rosa Gilson was red carded for the hosts.
Gremio celebrated an away win over Corinthians thanks to Douglas' solitary 34th-minute strike.
They held their one-goal lead for 32 minutes with 10 men following Junior Vilson's dismissal.
Vitoria drew 2-2 at Flamengo, Kleberson converting twice for the home side to cancel out efforts from Junior and Schwenck.