A Marek Jarolim goal earned Jablonec a 1-0 victory at Olomouc and a place at the top of the table.
Jarolim struck with just two minutes remaining to give Jablonec a one-point advantage over nearest challengers Teplice.
Pribram eased past struggling Ceske Budejovice 3-1 thanks to efforts from Jakub Stochl, Filip Dort and Rodriguez Da Silva. Zdenek Ondrasek netted the only reply for the visitors.
A last-minute penalty from Ondrej Szabo allowed Kladno to snatch a 3-2 win at home to Bohemians Prague.
Dimitri Tatanasvili and Lukas Zoubele were also on the scoresheet for Kladno, with Marek Kincl and Stanley Ibe getting the goals for Bohemians.